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錨點 2
錨點 1
  • Wu, C. C., Lin, C. C., Yin, H. Y., Tsai, W. C., Yang, P. F., Liu, H. J., & Wen, H. W*. 2024. Rapid detection of mango allergen in processed foods using an immunomagnetic nanoparticle-based electrochemical immunosensor. Microchemical Journal. 198: 110070. https://doi.org/10.1016/j.microc.2024.110070

  • Hsu, C. Y., Jang, Y., Huang, W. R., Wang, C. Y., Wen, H. W., Tsai, P. C., ... & Liu, H. J. 2023. Development of Polycistronic Baculovirus Surface Display Vectors to Simultaneously Express Viral Proteins of Porcine Reproductive and Respiratory Syndrome and Analysis of Their Immunogenicity in Swine. Vaccines. 11(11): 1666.

  • Wu, C. C., Singh, K., Ye, J. X., Chuang, Y. S., & Wen, H. W. 2023. A microfluidic chip integrating electrochemical impedimetric immunosensors constructed by top-bottom opposite electrodes for rapid detection of peanut allergen-Ara h 1. ​Sensors and Actuators B: Chemical. 396: 134637. https://doi.org/10.1016/j.aca.2015.04.041

  • Yin, H. Y., Liu, Y. F., Lin, Y. Y., Kao, T. C., Tsai, W. C., & Wen, H. W*. 2023. Development of a TaqMan real-time PCR assay with an internal amplification control for detecting trace amounts of allergenic mango (Mangifera indica) in commercial food products.European Food Research and Technology, 249(10):2647-2660.

  • W.C. Tsai, H.Y. Yin, W.T. Leong, C.Y. Tseng, H.W. Wen*. 2022. Development of an immunomagnetic nanoparticle-based lateral flow assay for detecting major kiwi allergen Act d1 in processed foods. European Food Research and Technology, 248(6), 1601-1613. https://link.springer.com/article/10.1007/s00217-022-03988-y

  • W.C. Tsai, T.L. Hoe, S.C. Lu, T.C. Kao, H.Y. Yin, J.W. Liao, H.W. Wen*. 2022. Lignosus rhinocerus attenuates non-alcoholic fatty liver induced by plant-based high-fat diet in hamster. Electronic Journal of Biotechnology. 58: 46–54. https://doi.org/10.1016/j.ejbt.2022.05.004

  • H. Y. Yin, W.C. Tsai, H.Y. Dai, H.W. Wen*. 2022. An immunochromatographic assay utilizing magnetic nanoparticles to detect major peanut allergen Ara h 1 in processed foods. Food Chemistry, 131844. https://doi.org/10.1016/j.foodchem.2021.131844

  • W.C. Tsai, H.Y. Yin, S.N. Y.T. Lin, H.J. Liu, C.Y. Tseng, H.W. Wen*. 2021. A rapid lateral flow assay using immunomagnetic nanoparticles for detecting mango allergen residues in processed foods. Journal of Food Safety. e12929. https://doi.org/10.1111/jfs.12929

 

  • W.C. Tsai, H.Y. Yin, S.N. Chen, H.C. Chang, H.W. Wen*. 2021. Development of monoclonal antibody-based sandwich ELISA for detecting major mango allergen Man i1 in processed foods. Journal of Food Safety. e12884. https://doi.org/10.1111/jfs.12884

  • W.C. Tsai, H.C. Chang, Y, H.Y. Yin, M.C. Huang, D.C. Agrawal, H.W. Wen*. 2020. The protective ability and cellular mechanism of Koelreuteria henryi Dummer flower extract against hydrogen peroxide-induced cellular oxidative damage. Electronic Journal of Biotechnology. 47: 89-99. https://doi.org/10.1016/j.ejbt.2020.07.006

 

  • W.C. Tsai, H.C. Chang, Y.H. Tseng, H.Y. Yin, J.W. Liao, D.C. Agrawal, H.W. Wen*. 2020. Toxicity evaluation of water extract of tissue-cultured Taraxacum formosanum by acute, subacute administration, and Ames test. Electronic Journal of Biotechnology, 45: 38–45. https://doi.org/10.1016/j.ejbt.2020.04.001

 

  • H.Y. Yin, Y.Y. Lin, C.H. Lin, W.C. Tsai, H.W. Wen*. 2019. Rapid and sensitive detection of Staphylococcus aureus in processed foods using a field‐deployed device to perform an insulated isothermal polymerase chain reaction‐based assay. Journal of Food Safety, 39(6): 1-8. https://doi.org/10.1111/jfs.12690

 

  • H.Y. Yin, T.J. Fang, Y.T. Li, YF Fung, W.C. Tsai, H.Y. Dai, H.W. Wen*. 2019. Rapidly detecting major peanut allergen-Ara h2 in edible oils using a new immunomagnetic nanoparticle-based lateral flow assay. Food Chemistry, 271: 505-515. NSC 104-2627-B-005-002. https://doi.org/10.1016/j.foodchem.2018.07.064

 

  • H.Y. Yin, H.W. Wen*. 2017. Dual-labeled PCR-based immunofluorescent assay for the rapid and sensitive detection of enterotoxic Staphylococcus aureus using cocktail sized liposomal nanovesicles as signal enhancer. Food Analytical Methods. 10 (10): 3264–3274.

  • W.C. Tsai, T.C. Wu, B.L. Chiang, H.W. Wen*. 2017. Cloning, expression, and purification of recombinant mango allergen Mangifera indica 1 in Escherichia coli. Protein Expression and Purification. 130: 35-43.

  • H.Y. Yin, T.J. Fang, H.W. Wen*. 2016. Combined multiplex loop-mediated isothermal amplification with lateral flow assay (m-LAMP/LFA) to detect sea and seb genes of enterotoxic Staphylococcus aureus. Letters in Applied Microbiology. 63(1):16-24.

 

  • H.Y. Yin, P.T. Chu, W.C. Tsai, H.W. Wen*. 2016. Development of a barcode-style lateral flow immunoassay for the rapid semi-quantification of gliadin in foods. Food Chemistry. 192: 934-942.

 

  • C.C. Li, H.P. Chung, H.W. Wen, C.T. Chang, Y.T. Wang, F.I. Chou. 2015. The radiation resistance and cobalt biosorption activity of yeast strains isolated from the Lanyu low-level radioactive waste repository in Taiwan. Journal of Environmental Radioactivity. 146: 80-87.

 

  • P.T. Chu., H.W. Wen*. 2013. Sensitive detection and quantification of gliadin contamination in gluten-free food with immunomagnetic beads based liposomal fluorescence immunoassay. Analytica Chimica Acta. 787: 246-253.

 

  • Chen, T.Y., Chen, D.Y., Wen, H.W., Ou, J.L., Chiou, S.S., Chen, J.M., Wong, M.L., Hsu, W.L. 2013. Inhibition of Enveloped Viruses Infectivity by Curcumin. PLOS ONE. 8:1-11.

 

  • Y.C. Lin, J.Y. Kuo, C.C. Hsu, W.C. Tsai, W.C. Li, M.C. Yu, H.W. Wen*. 2013. Optimizing manufacture of liposomal berberine with evaluation of its antihepatoma effects in a murine xenograft model. International Journal of Pharmaceutics. 441: 381-388.

 

  • P.T. Chu, C.S. Lin, W.J. Chen, C.F. Chen, H.W. Wen*. 2012. Detection of Gliadin in Foods Using a Quartz Crystal Microbalance Biosensor That Incorporates Gold Nanoparticles. Journal of Agricultural and Food Chemistry. 60: 6483-6492. 

 

  • W.C. Tsai, W.C. Li, H.Y Yin, M.C. Yu, H.W. Wen*. 2012. Constructing liposomal nanovesicles of ginseng extract against hydrogen peroxide-induced oxidative damage to L929 cells. Food Chemistry. 132:744-751.

 

  • C.H. Lin, F.Y. Tsai, W.L. Tsai, H.W. Wen, M.L. Hu. 2012. The feasibility of QR-code prescription in Taiwan Journal of Clinical Pharmacy and Therapeutics, 37: 643–646.

 

  • H. W.Wen, M. F. Hsieh, Y. T.Wang, et. al. 2010. Application of Gamma Irradiation in Ginseng for Both Photodegradation of Pesticide Pentachloronitrobenzene and Microbial Decontamination. Journal of Hazardous Materials. 176(1-3):280-7.

 

  • M.F. Hsieh, H.W. Wen, C.L. Shyu, et al. 2010. Synthesis, In-vitro Macrophage Response and Detoxification of Bamboo Charcoal Beads for Blood Purification. J Biomed Mater Res A. 94(4):1133-40.

 

  • H.T. Chou, H.W. Wen, T.Y. Kuo, et al. 2010. Interaction of cationic antimicrobial peptides with phospholipid vesicles and their antibacterial activity. Peptides. 31(10):1811-20.

 

  • P.T. Chu, S.Y. Yin, H.W. Wen*. 2009. Development of a rapid and sensitive immunomagnetic-bead based assay for detection of Bacillus cereus in milk. European Food Research and Technology. 229:73-81.

METHOD OF DETECTING MANGO ALLERGEN

Two methods of detecting mango allergen, SYBR Green qPCR and Taqman qPCR, each comprises: extracting a DNA of a sample; performing real-time PCR with the DNA of the sample and a first primer and a second primer to amplify a mango allergen man i1 gene, and detecting the amplified mango allergen man i1gene. Applying the method of the present application to allergen detection of foods may avoid a person who is allergic to the food from mistaking.

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